Yes!
Open image-widget.php and go to line 193 and replace it with this:
echo '<a class="'.$this->widget_options['classname'].'-image-link" rel="lightbox" href="'.$instance['link'].'" target="'.$instance['linktarget'].'">';
So basically just add rel=”lightbox”, and thats all. I’m using it with Lightbox Plus, but it should work with all versions.