I am having a similar issue. I no longer have the original file names but when I ran a test here – https://tools.pingdom.com/fpt/ – there would be one .png file that would take a very long time to load.
I replaced that file and it happened again. I replaced that file, and it’s currently looking for file 828315809.png
Permissions on that directory (top to bottom) is 755. I recreated the folder just to make sure it was working properly and the new empty folder produced files, but is still looking for a file that doesn’t exist.
Ideas please?