Most efficient method of calling content

Hey, so I have a general question about wordpress and PHP. I know my way around the basics of PHP but recently I have been wondering about something. I make a lot of templates and constantly find myself using the same functions multiple times in a single template file, for example ‘the_title()’. Would it be more efficient to use the function once at the top of the file and store the returned value in a variable, and then just call that variable – or does it not really make a difference?